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Two novel kits for the non-radioactive study of RNA-protein interactions
July 2010
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Thermo Fisher Scientific Inc.  
Thermo Fisher Scientific Inc. recently launched the LightShift RNA EMSA Kit and the Thermo Scientific Pierce RNA 3' End Biotinylation Kit for research into RNA-protein interactions using non-radioactive methods. The kit is the first commercially available kit for research using RNA electrophoretic mobility shift assay (EMSA). The kit uses a biotinylated RNA fragment to probe protein samples. RNA-protein complexes are then monitored by changes in electrophoretic migration patterns. To detect these changes, the kit uses chemiluminescent detection methods that provide comparable sensitivity to radioactive detection. The kit includes all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.  
 
Thermo Fisher Scientific Inc.
(781) 622-1000
 www.thermofisher.com
 

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