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High Throughput Screening NEWS
2018-06-12
  
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2018-06-12
  
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2018-02-14
  
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BMG Labtech
 
The PHERAstar® FSX is our latest, most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/Vis spectrometer, and dedicated AlphaScreen® and TRF Lasers, whatever your application, the PHERAstar FSX will perform it with ease and speed, even in 3456-well microplates.
 
BMG Labtech
 
GPCRs are important drug targets requiring receptor-protein interaction and trafficking studies to reveal how they function. Bioluminescence resonance energy transfer (BRET) is a versatile tool to study receptor biology. Its limitation of ectopic expression of labelled interaction partners is now overcome by CRISPR/Cas9 genome editing.
 
The CRISPR/Cas9 technique successfully fused the Nluc® BRET donor to endogenously-expressed CXCR4. The resulting protein levels were sufficient to monitor receptor interactions as well as internalization. The highly sensitive microplate readers by BMG LABTECH easily measured these low signal NanoBRET™ assays.
 
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