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Multi-blocking Morpholinos for miRNA Knockdowns, Gene Tools, LLC
July 2006
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MicroRNAs suppress expression of many genes, but existing tools for blocking miRNA function have not been optimal. Gene Tools has introduced custom-synthesized chemical tools to thoroughly block any selected miRNA. Using a new targeting technique, a single Morpholino oligo can block multiple steps in the maturation and activity of an miRNA, strongly knocking down its function. A steric-blocking Morpholino antisense oligo can block Drosha cleavage, Dicer cleavage, RISC loading and the ability of the mature miRNA to bind to its target. By lengthening a Morpholino to 31 bases, one end of the oligo can nucleate pairing in the single-stranded loop of a primary or pre-miRNA, facilitating invasion of the double-stranded miRNA stem, while the rest of the Morpholino extends across the miRNA sequence and overlaps the Drosha cleavage site. Gene Tools' Multi-blocking Morpholinos provide a convenient tool to knock down miRNA activity to help determine the roles of an miRNA in biological processes. Gene Tools manufactures custom-sequence Morpholino oligos for gene knockdown, splice modification and miRNA knockdown, as well as ancillary products for cellular delivery of Morpholinos. Publications describing experiments using Morpholino oligos are available at pubs.gene-tools.com.
 
Gene Tools
541.929.7840
www.gene-tools.com

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